Size

100 assays

Catalog no#

K4805-100

Price

909 EUR

Kit's other name

none

Species reactivity

human

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Category

Carcinoembryonic Antigen (CEA)

Samples tested

• Serum & plasma • Cell culture supernatants • Urine

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Antigens are peptides or recombinant or native dependent on the production method.

Applications

Quantitative protein detection, establishing normal range, validation of antibody array results

Highlights

• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

Contents

• CEA Ab-coated Microplate (Item A), 96 wells • Wash Buffer (20x) (Item B) • Human CEA Standard (Item C) • Assay Diluent A (Item D) • Assay Diluent B (5x) (Item E) • Detection Antibody (Item F) • HRP-Streptavidin (1000x) (Item G) • TMB (Item H) • Stop Solution (Item I)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Kit's benefits

• Easy, convenient and time-saving method to assay CEA. • The minimum detectable concentration of CEA is less than 200 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.

Kit's description

BioVision’s Human Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CEA is typically less than 200 pg/ml. Detection Range: 0.2 ng/ml – 250 ng/ml. The intra-Assay reproducibility is CV