Size
100 assays
Catalog no#
K4803-100
Price
909 EUR
Kit's other name
none
Species reactivity
human
Disease
cancer
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Category
Cancer Antigen 125 (CA-125)
Samples tested
• Serum & plasma • Cell culture supernatants • Urine
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description
Antigens are peptides or recombinant or native dependent on the production method.
Applications
Quantitative protein detection, establishing normal range, validation of antibody array results
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Contents
• CA-125 Ab-coated Microplate (Item A), 96 wells • Wash Buffer (20x) (Item B) • Human CA-125 Standard (Item C) • Assay Diluent (5x) (Item E) • Detection Antibody (Item F) • HRP-Streptavidin (800x ) (Item G) • TMB (Item H) • Stop Solution (Item I)
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Kit's benefits
• Easy, convenient and time-saving method to assay CA-125. • The minimum detectable concentration of CA-125 is less than 0.6 U/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.
Kit's description
BioVision’s Human Cancer Antigen 125 (CA-125) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-125. This assay employs an antibody specific for human CA-125 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA-125 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA-125 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA-125 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-γ, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of CA-125 is typically less than 0.6 U/ml. Detection Range: 0.6 U/ml – 400 U/ml. The intra-Assay reproducibility is CV