Size
200ug
Catalog no#
CSB-EP018840HU-200ug
Price
560 EUR
Tissue
cell, prostate, stem
Notes
For research use only.
Additional_information
MW: 35.2 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Description
Recombinant Human Prostate stem cell antigen(PSCA) expressed in E.coli
Additional description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Specifications
Uniprot ID: O43653; Host: Homo sapiens (Human); Expression system: E.coli; Expression region: 21-95aa ; Sequence information: Full Length of Mature Protein; Tag: N-terminal GST-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Storage_and_shipping
Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.