Size
96 Tests
Catalog no#
EKD01056
Price
349 EUR
Cross Activity
NA
Target's alterntive name
NA
Target Name
NA
Research Area
NA
Specificity
NA
Stability
NA
Tissue
total
Assay Time
2 hours
Assay Type
Sandwich
Sensitivity
0.1 ng/ml
Sample Type
Human Serum
Detection Range
0-100 ng/ml
Detection Method
Colorimetric
Shipping Conditions
Ambient shipping
Estimated Turnaround Time
4-7 business days
Species Reactivity
Homo sapiens (Human)
Application
For research use only. Not for diagnostic procedures.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality Systems
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Shelf life
Use Human Prostatic Specific Antigen - Total (PSA) ELISA Kit before 1 year
Description
Antigens are peptides or recombinant or native dependent on the production method.
Precision
The intra assay precision is between a CV% of 4.6-6.1 ng/ml. The inter assay precision is between a CV% of 3.8-9.5 ng/ml.
Storage Temperature
4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test Principle
The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of total PSA in the sample. A set of standards is used to plot a standard curve from which the amount of total PSA in patient samples and controls can be directly read.