Size
96 Tests
Catalog no#
EKE61768
Price
759 EUR
Research Area
NA
Precision
NA
Stability
NA
Specificity
NA
Target Name
NA
Target's alterntive name
Qa-2
Species Reactivity
Mouse
Assay Type
Sandwich
Assay Time
4.5 hours
Shipping Conditions
Ice packs
Sensitivity
0.19 ng/mL
Latin name
Mus musculus
Detection Method
Colorimetric
Detection Range
0.31-20 ng/mL
Estimated Turnaround Time
6-11 business days
Tissue
lymphocyte, region
Sample Type
Serum, Plasma, Biological Fluids
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Cross Activity
No significant cross-reactivity or interference was observed.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Mouse Qa-2 (Qa Lymphocyte Antigen 2 Region) ELISA Kit before 6 months
Description
Antigens are peptides or recombinant or native dependent on the production method.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Qa-2 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Qa-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Qa-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Qa-2. The concentration of Mouse Qa-2 in samples can be calculated by comparing the OD of the samples to the standard curve.