Size
96 Tests
Catalog no#
EKU06866
Price
885 EUR
Target's alterntive name
NA
Assay Time
3 hours
Shipping Conditions
Ice packs
Sensitivity
0.063 ng/mL
Detection Method
Colorimetric
Research Area
Tumor immunity
Detection Range
0.156-10 ng/mL
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Homo sapiens (Human)
Tissue
cell, prostate, stem
Assay Type
Double-antibody Sandwich
Target Name
Prostate Stem Cell Antigen
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Sample Type
Tissue homogenates, Cell lysates and other biological fluids.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Human Prostate Stem Cell Antigen (PSCA) ELISA Kit before 8 months
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Specificity
This assay has high sensitivity and excellent specificity for detection of Prostate Stem Cell Antigen
Cross Activity
No significant cross-reactivity or interference between Prostate Stem Cell Antigen and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Prostate Stem Cell Antigen and analogues was observed.
Description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Stem Cell Antigen were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Stem Cell Antigen were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.