Size
10ug
Catalog no#
PAC735Hu01
Price
255 EUR
Alternative Names
-
Image number
2
Species reactivity
Human
Aplication
WB,IHC
Concentration
500ug/ml
French translation
anticorps
Clonality
Rabbit polyclonal
Delivery condition
4℃ with ice bags
Sequence of immunogen
PSCA (Leu22~Leu123)
Organism Species
Homo sapiens (Human)
Tissue
cell, prostate, stem
Latin name
Oryctolagus cuniculus
Group
Polyclonals and antibodies
Item Name
Prostate Stem Cell Antigen
Source
Polyclonal antibody preparation
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Immunogen
RPC735Hu01-Recombinant Prostate Stem Cell Antigen (PSCA)
Applicable Secondary Antibody
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.