antigenetics.com

500ul

BMDV10152

808 EUR

cells

anticorps

Monoclonal Antibody

Mus musculus,Rattus norvegicus

Antigens are peptides or recombinant or native dependent on the production method.

Melan-A MART-1 Melanoma Antigen Recognized by T-cells 1 Melanoma Marker 18kD Clone: M2-7C10 Horse NO X w/ or /flow denatured

anti-Melan-A MART-1 Melanoma Antigen Recognized by T-cells 1 Melanoma Marker 18kD Clone: M2-7C10 Horse NO X w/ or /flow denatured

Melan-A, MART-1 (Melanoma Antigen Recognized by T-cells 1), Melanoma Marker, 18kD, Clone: M2-7C10, Mab anti-Human, Horse, NO X w/Mouse or Rat; paraffin, IH/WB/flow/IP (denatured)

Melan-A, MART-1 (Melanoma Antigen Recognized by T-cells 1), Melanoma Marker, 18kD, Clone: M2-7C10, Mab antibody-Human, Horse, NO X w/Mouse or Rat; paraffin, IH/WB/flow/IP (denatured)

Melan-A, MART-1 (Melanoma Antigen Recognized by T-cells 1), Melanoma Marker, 18kD, Clone: M2-7C10, Mab antibody-Homo sapiens, Horse, NO X w/Mus musculus or Rattus norvegicus; paraffin, IH/WB/flow/IP (denatured)

To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.

Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.

If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.