Size
vial
Catalog no#
YSRTMCA497A405
Price
5 EUR
French translation
anticorps
Conjugation
Alexa Fluor
Clonality
Monoclonal Antibody
Latin name
Mus musculus,Rattus norvegicus
Alternative name 5
F4/80 Antigen Macrophage mature 160kD Clone: C1:A3-1 ALEXA 405
Alternative name 4
anti-F4/80 Antigen Macrophage mature 160kD Clone: C1:A3-1 ALEXA 405
Additional description
Antigens are peptides or recombinant or native dependent on the production method.
Alternative name 1
F4/80 Antigen, Macrophage (mature), 160kD, Clone: C1:A3-1, Rat Mab anti-Mouse, ALEXA 405 conj.
Alternative name 2
F4/80 Antigen, Macrophage (mature), 160kD, Clone: C1:A3-1, Rat Mab antibody-Mouse, ALEXA 405 conj.
Alternative name 3
F4/80 Antigen, Macrophage (mature), 160kD, Clone: C1:A3-1, Rattus norvegicus Mab antibody-Mus musculus, ALEXA 405 conj.
Properties
For facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.