Size

10x96T

Catalog no#

EKU07450

Price

5877 EUR

Verified applications

ELISA

Protein number

Q86W05

Assay duration

3 hours

Use for

8 months

Assay class

Sandwich

Shipping requirements

Blue ice

Sensitivity limit

0,055 ng/mL

ELISA detection

Colorimetric

Detection limits

0.156-10 ng/mL

Tissue

carcinoma, cell

Estimated production time

7-11 business days

Verified reactivity

Homo sapiens (Human)

Gene number

Please refer to GenBank

Alternate protein number

Please refer to SwissProt

Research main area

Tumor immunity;Gastroenterology;

Gene name

Squamous Cell Carcinoma Antigen 2

Protocol

Please see ELISA's datasheet, otherwise contact us

Notes

For research use only. Not for diagnostic procedures.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Alternate gene name

SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor,Clade B,Member 4; Peptidase Inhibitor 11

Samples to be analyzed

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Warnings

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 2 (SCCA2). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 2 (SCCA2) and analogues was observed.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 2 (SCCA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 2 (SCCA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Assay principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 2 (SCCA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Squamous Cell Carcinoma Antigen 2 (SCCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Squamous Cell Carcinoma Antigen 2 (SCCA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.