Size

10x96T

Catalog no#

EKU08939

Price

7723 EUR

Gene number

6317

Verified applications

CLIA

Protein number

P29508

Shipping requirements

Blue ice

Use for

8 months

Assay class

Sandwich

Sensitivity limit

5,63 pg/mL

Assay duration

2 hours, 40min

Tissue

carcinoma, cell

ELISA detection

Chemiluminescent

Estimated production time

7-11 business days

Detection limits

156.25-10000 pg/mL

Verified reactivity

Homo sapiens (Human)

Alternate protein number

Please refer to SwissProt

Gene name

Squamous Cell Carcinoma Antigen 1

Research main area

Tumor immunity;Pulmonology;Hepatology;

Protocol

Please see ELISA's datasheet, otherwise contact us

Notes

For research use only. Not for diagnostic procedures.

Samples to be analyzed

Serum, plasma, tissue homogenates and other biological fluids.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Alternate gene name

SERPINB3; T4-A; Serpin Peptidase Inhibitor,Clade B(ovalbumin),Member 3; Serpin B3

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Warnings

Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 1 (SCCA1). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 1 (SCCA1) and analogues was observed.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay principle

The microplate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Squamous Cell Carcinoma Antigen 1 (SCCA1) level in the sample or standard.;

Description

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.