antigenetics.com

10x96T

EKD01056

2251 EUR

total

ELISA

1 year

2 hours

Biology

Sandwich

0,1 ng/ml

Human Serum

0-100 ng/ml

Colorimetric

Ambient shipping

4-7 business days

Homo sapiens (Human)

Please refer to NCBI

Please refer to GenBank

Please refer to Uniprot

Please refer to GenBank

Please refer to SwissProt

Please see ELISA's datasheet, otherwise contact us

Please see ELISA's datasheet, otherwise contact us

For research use only. Not for diagnostic procedures.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

To know the specificity of this test please feel free to contact us

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

The intra assay precision is between a CV% of 4.6-6.1 ng/ml. The inter assay precision is between a CV% of 3.8-9.5 ng/ml.

4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Antigens are peptides or recombinant or native dependent on the production method.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of total PSA in the sample. A set of standards is used to plot a standard curve from which the amount of total PSA in patient samples and controls can be directly read.