Size

10x96T

Catalog no#

EKU02910

Price

7084 EUR

Verified applications

ELISA

Assay duration

2 hours

Research main area

Biology

Shipping requirements

Blue ice

Use for

8 months

Sensitivity limit

0,86 ng/mL

Assay class

Competitive

ELISA detection

Colorimetric

Detection limits

2.47-200 ng/mL

Estimated production time

7-11 business days

Verified reactivity

Homo sapiens (Human)

Alternate gene name

Please refer to NCBI

Protein number

Please refer to Uniprot

Gene number

Please refer to GenBank

Gene name

Carbohydrate Antigen 19-5

Alternate protein number

Please refer to SwissProt

Samples to be analyzed

Serum, plasma and other biological fluids.

Protocol

Please see ELISA's datasheet, otherwise contact us

Notes

For research use only. Not for diagnostic procedures.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Carbohydrate Antigen 19-5 (CA19-5). No significant cross-reactivity or interference between Carbohydrate Antigen 19-5 (CA19-5) and analogues was observed.

Warnings

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbohydrate Antigen 19-5 (CA19-5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbohydrate Antigen 19-5 (CA19-5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

Antigens are peptides or recombinant or native dependent on the production method.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Assay principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carbohydrate Antigen 19-5 (CA19-5) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carbohydrate Antigen 19-5 (CA19-5) and unlabeled Carbohydrate Antigen 19-5 (CA19-5) (Standards or samples) with the pre-coated antibody specific to Carbohydrate Antigen 19-5 (CA19-5). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample.