Size

100ug

Catalog no#

GEN604019

Price

691 EUR

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Concentration

N/A

Clone

5D3

Other names

N/A

Immunoglobulin isotype

IgG1k

French translation

anticorps

Category

Antibodies

Clonality

Monoclonal

Tissue

lymphocyte

Conjugation

Biotinylated

Also known as

CD152, Blocking

Source organism

Human (Homo sapiens)

Host organism

Mouse (Mus musculus)

Subcategory

Mnoclonal antibodies

Purification method

Affinity Purified by Protein A affininty chromatography.

Test

You can block the antibody by the specific target amino acid sequence of peptide.

Description

Antigens are peptides or recombinant or native dependent on the production method.

Tested applications:

ELISA (EL/EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Form/Appearance

Supplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Specificity and cross-reactivity

Recognizes an extracellular epitope of human CTLA-4 expressed on activated T cells. Blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor. Detects immobilized recombinant CD152 IgG2a Fc fusion protein in ELISA.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Properties

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.