Size

100 Tests

Catalog no#

GEN213569

Price

299 EUR

Gene name synonims

N/A

Purification method

N/A

Concentration

N/A

Immunoglobulin isotype

IgG2a

Clone

PK136

Gene name

CD161b/c

Also known as

CD161b/c

French translation

anticorps

Clonality

Monoclonal

Category

Antibodies

Latin name

Mus musculus

Host organism

Mouse (Mus musculus)

Subcategory

Mnoclonal antibodies

Tested applications:

Flow Cytometry (FACS)

Other gene names

N/A; Klrb1b; N/A; Ly55b; Nkrp1b; Ly-55b; NKR-P1B

Form/Appearance

RPE (Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised)

Properties

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.

Other names

Killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele A; N/A; N/A; CD161 antigen-like family member B; Lymphocyte antigen 55b; Ly-55b; NKR-P1 34; Natural killer cell surface protein NKR-P1B allele SJL/BALB

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Storage and shipping

Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. Following reconstitution Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of reconstitution.

Specificity and cross-reactivity

CD161b/c This item (clone PK136) recognises the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family.In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This item is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Clone PK136 has also been reported to be suitable for in vivo depletion of NK cells and in vitro activation of NK cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.