Size
200ug
Catalog no#
GEN633035
Price
696 EUR
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Immunoglobulin isotype
IgM
Other names
N/A
Clone
1.BB.220
Clonality
Monoclonal
Category
Antibodies
Conjugation
Biotinylated
Also known as
CD15, Granulocytes
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Tested applications:
Flow Cytometry (FC/FACS)
Purification method
Highly Purified by Ion Exchange chromatography.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Form/Appearance
Supplied as a liquid, in PBS, pH7.2 containing 1% BSA, 0.1% Sodium Azide. Label with Biotin.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Specificity and cross-reactivity
Recognizes a (Mr 156kD) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes. No reactivity with T or B cells (1,2,3).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.