Size

100ug

Catalog no#

GEN632688

Price

906 EUR

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Concentration

N/A

Immunoglobulin isotype

IgG1

Also known as

CD229

Purification method

Purified

Clone

HLy9.1.25

Category

Antibodies

Clonality

Monoclonal

Tissue

cell, lymphocyte

Conjugation

Anti-FITC Antibody

Host organism

Mouse (Mus musculus)

Subcategory

Mnoclonal antibodies

Source organism

Human (Homo sapiens)

Other names

cytochrome oxidase subunit I; N/A

Tested applications:

Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Form/Appearance

Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

Recognizes human CD229at ~100-120kD. Species sequence homology: mouse.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Description

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.

Properties

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.