Size
100 Tests
Catalog no#
GEN604171
Price
669 EUR
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Other names
N/A
Immunoglobulin isotype
IgG2b
Clone
3H1355
Also known as
CD44V7, 8
Category
Antibodies
Clonality
Monoclonal
Purification method
Purified IgG.
Tissue
cell, lymphocyte
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Source organism
Human (Homo sapiens)
Tested applications:
Flow Cytometry (FC/FACS)
Storage and shipping
Store the antibody at -20 degrees Celsius.
Form/Appearance
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid in Tris buffered saline with 0.02% sodium azide, 1% BSA.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Specifically recognizes an epitope encoded by exons v7-v8 on the variant portion of human CD44.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the CD44V7, 8 (HCAM, Homing Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (FITC).Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.