Size
100ug
Catalog no#
GEN604019
Price
691 EUR
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Clone
5D3
Immunoglobulin isotype
IgG1k
Category
Antibodies
Tissue
lymphocyte
Clonality
Monoclonal
Conjugation
Biotinylated
Also known as
CD152, Blocking
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Host organism
Mouse (Mus musculus)
Purification method
Affinity Purified by Protein A affininty chromatography.
Test
You can block the antibody by the specific target amino acid sequence of peptide.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Tested applications:
ELISA (EL/EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Form/Appearance
Supplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Specificity and cross-reactivity
Recognizes an extracellular epitope of human CTLA-4 expressed on activated T cells. Blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor. Detects immobilized recombinant CD152 IgG2a Fc fusion protein in ELISA.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.