Size

1000ug

Catalog no#

GEN601676

Price

1149 EUR

Concentration

N/A

Immunoglobulin isotype

IgG1

Clone

3H1050

Category

Antibodies

Clonality

Monoclonal

Host organism

Mouse (Mus musculus)

Subcategory

Mnoclonal antibodies

Source organism

Human (Homo sapiens)

Also known as

CD18, Activation Epitope

Other names

CD18; N/A; CD18; N/A; N/A

Other gene names

LOC100304487; N/A; N/A; N/A

Gene name synonims

LOC100304487; N/A; N/A; N/A

Gene name

LOC100304487; N/A; N/A; N/A

Form/Appearance

Supplied as a liquid. Azide free

Purification method

Affinity Purified by Protein G affinity chromatography.

Tested applications:

Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Description

Antigens are peptides or recombinant or native dependent on the production method.Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request.Recently MBS Monoclonals made this CD18, Activation Epitope (Leukocyte Associated Antigen, LFA-1 beta2) ( ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.

Specificity and cross-reactivity

Recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone 3H1050 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized is also exposed during dissociation of the CD11/CD18 by low pH.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.