Size

100ug

Catalog no#

GEN601133

Price

658 EUR

Concentration

N/A

Immunoglobulin isotype

IgG1

Clone

3H1050

Category

Antibodies

Clonality

Monoclonal

Conjugation

Biotinylated

Subcategory

Mnoclonal antibodies

Host organism

Mouse (Mus musculus)

Source organism

Human (Homo sapiens)

Tested applications:

Flow Cytometry (FC/FACS)

Also known as

CD18, Activation Epitope

Other names

CD18; N/A; CD18; N/A; N/A

Other gene names

LOC100304487; N/A; N/A; N/A

Gene name synonims

LOC100304487; N/A; N/A; N/A

Gene name

LOC100304487; N/A; N/A; N/A

Storage and shipping

Store the antibody at -20 degrees Celsius.

Purification method

Affinity Purified by Protein G affinity chromatography.

Form/Appearance

Purified IgG conjugated to Biot- liquid with 0.1% sodium azide, 1% BSA.

Description

Antigens are peptides or recombinant or native dependent on the production method.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Specificity and cross-reactivity

Recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone 3H1050 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by 3H1050 is also exposed during dissociation of the CD11/CD18 by low pH.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.