antigenetics.com

100 Tests

GEN601102

525 EUR

N/A

N/A

N/A

N/A

N/A

N/A

IgG1

6D31

CD44V3

Antibodies

Monoclonal

Biotinylated

cell, lymphocyte

Mouse (Mus musculus)

Mnoclonal antibodies

Human (Homo sapiens)

Store the antibody at -20 degrees Celsius.

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Liquid. 100 tests in 1ml TRIS, pH 7.4, containing 100mM NaCl, 1% BSA and 0.02% sodium azide.

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Recognizes an epitope encoded by exon v3 on the variant portion of human CD44.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the CD44V3 (HCAM, Homing Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) ( ).Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.