Size

500ug

Catalog no#

GEN600118

Price

542 EUR

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Concentration

N/A

Other names

N/A

Clone

36-7-5

Immunoglobulin isotype

IgG2a,k

Purification method

Purified

Category

Antibodies

Clonality

Monoclonal

Conjugation

Biotinylated

Also known as

MHC Class 1, H2, KK

Subcategory

Mnoclonal antibodies

Host organism

Mouse (Mus musculus)

Source organism

Mouse (Mus musculus)

Tested applications:

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Form/Appearance

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.

Species reactivity

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Description

The MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K) H2-K1, H2-K) ( ) is a α- or alpha protein sometimes glycoprotein present in blood.Antigens are peptides or recombinant or native dependent on the production method.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Specificity and cross-reactivity

Reacts with the H-2Kk MHC class I alloantigen. Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. Does not react with other (e.g., b, d, q) haplotypes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.