Gene name synonims
Other gene names
Also known as
MHC Class 1, H2, KK
Mouse (Mus musculus)
Mouse (Mus musculus)
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
The MHC Class 1, H2, KK (H-2 Class I Histocompatibility Antigen, K-K alpha Chain, H-2K(K) H2-K1, H2-K) (FITC) is a α- or alpha protein sometimes glycoprotein present in blood.Antigens are peptides or recombinant or native dependent on the production method.
Specificity and cross-reactivity
Reacts with the H-2Kk MHC class I alloantigen. Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. Does not react with other (e.g., b, d, q) haplotypes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.