Size
100ug
Catalog no#
11-489-C100
Price
223 EUR
Applications
WB
Concentration
1 mg/ml
Disease
neisseria
Clone
Polyclonal
Purity
> 95% (by SDS-PAGE)
Species reactivity
Neisseria meningitidis
Format/conjugation
purified; unconjugated
Research area
Virology and Microbiology
Clonality
Polyclonal Antibody (PAb)
Antigen
Neisseria meningitidis antigen Orf1 / FrpD
Purification method
Purified from rabbit serum by affinity chromatography
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Description
Antigens are peptides or recombinant or native dependent on the production method.
Long name
Rabbit Polyclonal Antibody against Neisseria meningitidis Neisseria meningitidis antigen Orf1 / FrpD, Clone: PAb
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
Immunogen
Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
References
*Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P: The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280(5):3251-8. Epub 2004 Nov 2. _x000D_
Specificity
The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
Background
Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Shipping and storage
Rabbit Polyclonal Antibody against Neisseria meningitidis Neisseria meningitidis antigen Orf1 / FrpD, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.