Size
25 assays
Catalog no#
1F-213-T025
Price
131 EUR
Antigen
CD15
Format/conjugation
FITC
Tissue
blood
Clone
MEM-158
French translation
anticorps
Applications
FC, IP, MC
Immunogen
Human granulocytes
Purity
> 95% (by SDS-PAGE)
Species reactivity
Human (Homo sapiens)
Negative species reactivity
Porcine, Bovine, Sheep
Clonality
Monoclonal Antibody (Mab)
Purification method
Purified by affinity chromatography
Research area
Blood Group Antigens (Human), CD and Related Antigens (Human)
References
*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Long name
Mouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158
Storage buffer
The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
Antigens are peptides or recombinant or native dependent on the production method.Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Exbio supplies this blood related product in 1.
Specificity
The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells._x000D_ HLDA VI; WS Code AS A053
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Shipping and storage
Mouse Monoclonal Antibody against Human CD15 / Lewis x Blood Group antigen, Clone: MEM-158 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
Background
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.