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Anti-Flavivirus group antigen Antibody

Catalog no#

A1147-200

Price

697 EUR
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Target of this antibody:

N/A

Alternative names of antibody target:

N/A

Form of antibody:

Liquid

Shipping conditions:

gel pack

French translation

anticorps

Clonality of antibody:

Monoclonal

Antibody host:

Recombinant

Clone:

D1-4G2-4-15 (4G2)

Isotype:

Mouse Ig2a, kappa

This antibody has been succesfully tested for use in following methods:

IF; WB; NTRL; ELISA

Immunogene sequence:

Dengue Virus type 2 antigens

Purification method:

Affinity purified using protein A

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

How to handle this antibody:

The antibody solution should be gently mixed before use

Formulation of this antibody:

200 µg affinity purified antibody in phosphate-buffered saline (PBS) containing 0.02% Proclin 300

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.

Background information:

This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation. The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004. Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008).