antigenetics.com

96-wells plate (breakable strips)

EK0104

859 EUR

1-4h

450 nm

hepatitis

Guinea pig

Enzyme Linked Immunosorbent Assay

hepatitis B virus surface antigen (HBsAg)

Cavia hepatitis B virus surface antigen (HBsAg) ELISA kit

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

ELISA test kit for Guinea pig hepatitis B virus surface antigen (HBsAg)

Pigs and the smaller guinea pigs are frequent used as models for humans.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This is a ready to use kit for performing ELISA tests for detection of hepatitis B virus surface antigen (HBsAg) in samples from Guinea pig

Our hepatitis B virus surface antigen (HBsAg) ELISA kit has been proven to work and successfully detect hepatitis B virus surface antigen (HBsAg) in Guinea pig

The hepatitis B virus surface antigen (HBsAg) ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Guinea pig hepatitis B virus surface antigen (HBsAg) ELISA kit is 6 months after delivery.

ELISA test kit for Guinea pig hepatitis B virus surface antigen (HBsAg)Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual

Due to transportation and handling small amounts of the liquid components of the Cavia hepatitis B virus surface antigen (HBsAg) ELISA kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.

1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.

Antigens are peptides or recombinant or native dependent on the production method.Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.Hepatitis a, b, c, d, e recombinant surface viral antigens to genotype 1, 2, 3, 4, 5 are used for antibody production to make diagnostic poly or monoclonal antibodies. Hepatitis is a disease of the liver characterized by the presence of inflammatory cells in the tissue of the organ. Hepatitis may occur without symptoms, but can lead to jaundice (a yellow discoloration of the skin, mucous membranes, and conjunctiva of the eyes), poor appetite, and fatigue. Depending on the cause, hepatitis can manifest either as an acute or as a chronic disease.