Size
96Test
Catalog no#
E-CL-H1082-96T
Price
541 EUR
UNIProt ID
NA
Target Synonym
NA
Target Name
SCCA2
Target Species
Human
Type
Sandwich
Sensitivity
18.75pg/mL
Detection Type
Colormetric
Tissue
carcinoma, cell
Detection Range
31.25~2000pg/mL
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Human SCCA2(Squamous Cell Carcinoma Antigen 2) CLIA Kit
Description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA2 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA2 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCCA2 . You can calculate the concentration of Human SCCA2 in the samples by comparing the RLU value of the samples to the standard curve.