Size
96Test
Catalog no#
E-CL-M0618-96T
Price
541 EUR
UNIProt ID
NA
Target Synonym
NA
Target Name
SCCA2
Target Species
Mouse
Type
Sandwich
Sensitivity
7.5pg/mL
Detection Type
Colormetric
Latin name
Mus musculus
Detection Range
12.5~800pg/mL
Tissue
carcinoma, cell
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Mouse SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit
Description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2 . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCCA2 , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SCCA2 . You can calculate the concentration of Mouse SCCA2 in the samples by comparing the RLU value of the samples to the standard curve.