Size
96Test
Catalog no#
E-CL-R0555-96T
Price
541 EUR
UNIProt ID
NA
Target Synonym
NA
Target Species
Rat
Tissue
cell
Target Name
PCNA
Type
Sandwich
Sensitivity
18.75pg/mL
Detection Type
Colormetric
Detection Range
31.25~2000pg/mL
Latin name
Rattus norvegicus
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit
Description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PCNA . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCNA , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PCNA . You can calculate the concentration of Rat PCNA in the samples by comparing the RLU value of the samples to the standard curve.