Size
96Test
Catalog no#
E-EL-H5413-96T
Price
492 EUR
Target Name
PSMA
Target Species
Human
UNIProt ID
Q04609
Tissue
prostate
Type
Sandwich
Sensitivity
2.81ng/mL
Detection Type
Colormetric
Detection Range
4.69~300ng/mL
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Human PSMA(Prostate specific membrane antigen) EIA Kit
Target Synonym
FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP, N-acetylated alpha-linked acidic dipeptidase 1|cell growth-inhibiting protein 27|folate hydrolase 1|folylpoly-gamma-glutamate carboxypep
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
Antigens are peptides or recombinant or native dependent on the production method.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Human PSMA. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSMA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSMA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PSMA. You can calculate the concentration of Human PSMA in the samples by comparing the OD of the samples to the standard curve.