Size
10µg
Catalog no#
PKSR030396-10µg
Price
210 EUR
Fusion tag
C-6His
Accession
O35531
Mol Mass
25.9 kDa
AP Mol Mass
35-55 kDa
Tissue
lymphocyte
ExpressedHost
Human cells
Sequence
Vla29-Lys247
Group
recombinants
Latin name
Rattus norvegicus
Source
Recombinants or rec. proteins
Purity
＞95% as determined by reducing SDS-PAGE.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Synonym
T-lymphocyte activation antigen CD86; Activation B7-2 antigen; CD86
Description
Antigens are peptides or recombinant or native dependent on the production method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-160 ng/ml.
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Background
T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. It is highly expressed on activated antigen presenting cells. CD86 involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.