Size
10µg
Catalog no#
PKSM040859-10µg
Price
156 EUR
Mol Mass
45.8 kDa
Fusion tag
C-Fc-6His
AP Mol Mass
60-66 kDa
Expressed Host
Human Cells
Group
recombinants
Latin name
Mus musculus
Sequence
Met 1-Arg 182
Accession
NP_001028298.1
Source
Recombinants or rec. proteins
Purity
＞95% as determined by reducing SDS-PAGE.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Synonym
CD27 antigen; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; CD27
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Background
Mouse CD27 is a single-pass type I membrane protein which is a member of the tumor necrosis factor receptor superfamily. CD27 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. CD27 transduces signals that lead to the activation of NF-κB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.