Size
50µg
Catalog no#
PKSM040679-50µg
Price
203 EUR
Fusion tag
C-6His
Accession
Q00609
Mol Mass
24.6 kDa
AP Mol Mass
38-55 kDa
Tissue
lymphocyte
Expressed Host
Human Cells
Sequence
Val38-Asn246
Group
recombinants
Latin name
Mus musculus
Source
Recombinants or rec. proteins
Purity
＞95% as determined by reducing SDS-PAGE.
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Synonym
T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80
Activity
Measured by its ability to bind recombinant mouse CD28 in a functional ELISA.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Background
Cluster of Differentiation 80, also called B7-1, is a member of cell surface immunoglobulin superfamily which plays key, yet distinct roles in the activation of T cells. Mouse CD80 consists of an extracellular domain (ECD) with twoimmunoglobulin-like domains, transmembrane segment, and cytoplasmic domain. B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T- and B- cell responses. CD80 is mostly expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response.