Size
10µg
Catalog no#
PKSH032703-10µg
Price
267 EUR
Fusion tag
C-Fc
AP Mol Mass
60 kDa
Accession
Q08722
Mol Mass
41.9 kDa
Expressed Host
Human cells
Group
recombinants
Sequence
Gln19-Pro139
Source
Recombinants or rec. proteins
Purity
＞95% as determined by reducing SDS-PAGE.
Endotoxin
＜1.0 EU per µg as determined by LAL test.
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB，150mM NaCl,pH7.4.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Activity
Measured by its ability to bind Human SIRPA in functional ELISA.The ED50 for this effect is less than 200 ng/ml.
Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Synonym
Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Background
CD47(Integrin-Associated Protein,IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.