Size

50ul

Catalog no#

BT-AP04540-50

Price

268 EUR

French translation

anticorps

Tissue

cell, prostate, stem

Latin name

Oryctolagus cuniculus

Group

Polyclonals and antibodies

Properties

If you buy Antibodies supplied by BT-Laboratory they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by BT-Laboratory. Rabbit antibodies are very stable and can be stored for several days at room temperature. BT-Laboratory adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Test

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.