Size
10x96T
Catalog no#
EKD01056
Price
2251 EUR
Tissue
total
Verified applications
ELISA
Use for
1 year
Assay duration
2 hours
Research main area
Biology
Assay class
Sandwich
Sensitivity limit
0,1 ng/ml
Samples to be analyzed
Human Serum
Detection limits
0-100 ng/ml
ELISA detection
Colorimetric
Shipping requirements
Ambient shipping
Estimated production time
4-7 business days
Verified reactivity
Homo sapiens (Human)
Alternate gene name
Please refer to NCBI
Gene number
Please refer to GenBank
Protein number
Please refer to Uniprot
Gene name
Please refer to GenBank
Alternate protein number
Please refer to SwissProt
ELISA's stability
Please see ELISA's datasheet, otherwise contact us
Protocol
Please see ELISA's datasheet, otherwise contact us
Notes
For research use only. Not for diagnostic procedures.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
ELISA's specificity
To know the specificity of this test please feel free to contact us
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Precision of the test
The intra assay precision is between a CV% of 4.6-6.1 ng/ml. The inter assay precision is between a CV% of 3.8-9.5 ng/ml.
Storage recommendation
4°C. Bring all reagents to room temperature before beginning test. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
Antigens are peptides or recombinant or native dependent on the production method.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Assay principle
The principle of the following enzyme immunoassay test follows a typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for total PSA is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of PSA is conjugated to horse radish peroxidase (HRP). Total PSA from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of total PSA in the sample. A set of standards is used to plot a standard curve from which the amount of total PSA in patient samples and controls can be directly read.