Size

NA

Catalog no#

MBS602398

Price

5 EUR

Reactivity

Human

Products_type

Antibody

Conjugation

Biotinylated

Tissue

cell, lymphocyte

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Description

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the CD44V7, 8 (HCAM, Homing Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) ( )[CD44V7, 8].Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.