Size
100ug
Catalog no#
GEN604449
Price
691 EUR
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Concentration
N/A
Immunoglobulin isotype
IgM,k
Clone
9H242
Also known as
HESCA-1
Purification method
Purified.
French translation
anticorps
Tissue
cell, stem
Clonality
Monoclonal
Category
Antibodies
Source organism
Human (Homo sapiens)
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Form/Appearance
Supplied as a liquid in PBS, 0.25 sodium chloride, pH 7.6, 0.1% sodium azide.
Tested applications:
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Description
Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes H1, BG-01, BG01v, and BF02 human embryonic stem (hES) cell lines. Unreactive for differentiated stem cells in teratomas and common feeder lines such as MEF, D-551, and Hs27. Reactivity with other hES lines has not yet determined.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.