Size
100ug
Catalog no#
GEN216751
Price
531 EUR
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Purification method
N/A
Other names
N/A
Immunoglobulin isotype
IgG2a
Clone
HCMV37
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Tested applications:
Immunofluorescence (IF)
Disease
Cytomegalovirus or human CMV
Also known as
CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Form/Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Species reactivity
Viral; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.To cause (an acid or base) to undergo neutralization.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CYTOMEGALOVIRUS gB LATE NEUTRALIZING ANTIGEN This item is specific for Cytomegalovirus glycoprotein B late neutralizing antigen._x000D_Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human herpesvirus 5 (HHV-5). _x000D__x000D_Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of approximately 100kDa. _x000D__x000D_This item reacts with MRC-5 infected cells but not uninfected cells. This antibody has been found to be neutralizing.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.