Size

200ug

Catalog no#

GEN633035

Price

696 EUR

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Concentration

N/A

Immunoglobulin isotype

IgM

Other names

N/A

Clone

1.BB.220

Clonality

Monoclonal

Category

Antibodies

Conjugation

Biotinylated

Also known as

CD15, Granulocytes

Host organism

Mouse (Mus musculus)

Subcategory

Mnoclonal antibodies

Source organism

Human (Homo sapiens)

Tested applications:

Flow Cytometry (FC/FACS)

Purification method

Highly Purified by Ion Exchange chromatography.

Description

Antigens are peptides or recombinant or native dependent on the production method.

Form/Appearance

Supplied as a liquid, in PBS, pH7.2 containing 1% BSA, 0.1% Sodium Azide. Label with Biotin.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Specificity and cross-reactivity

Recognizes a (Mr 156kD) cell surface glycoprotein expressed on virtually all peripheral blood granulocytes. No reactivity with T or B cells (1,2,3).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.