Size

0.25 ml

Catalog no#

GEN604222

Price

614 EUR

Concentration

N/A

Immunoglobulin isotype

IgG2b

Clone

0.L.1

Category

Antibodies

Clonality

Monoclonal

Conjugation

Anti-FITC Antibody

Subcategory

Mnoclonal antibodies

Host organism

Mouse (Mus musculus)

Source organism

Human (Homo sapiens)

Also known as

HLA Class 1 Antigen A9

Other gene names

HLA-A; HLA-A; HLAA; HLAA

Gene name synonims

HLA-A; HLA-A; HLAA; HLAA

Gene name

HLA-A; HLA-A; HLAA; HLAA

Purification method

Highly Purified by Ion Exchange chromatography.

Tested applications:

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Form/Appearance

Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Labeled with fluorescein isothiocyanate (FITC). Also available with BSA & Azide. See H6098-23A.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Description

Antigens are peptides or recombinant or native dependent on the production method.Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request.

Specificity and cross-reactivity

Reacts to HLA Class 1 Antigen-A9.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Other names

HLA-A9HH antigen; HLA class I histocompatibility antigen, A-68 alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze the antibody should be stored at -20 degrees Celsius.. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.

Properties

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.