Size
100ug
Catalog no#
GEN603071
Price
630 EUR
Concentration
N/A
Immunoglobulin isotype
IgG1
Clone
3H2215
Category
Antibodies
Clonality
Monoclonal
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Source organism
Human (Homo sapiens)
Also known as
HLA Class 1 Antigen B7
Tested applications:
Flow Cytometry (FC/FACS)
Other gene names
HLA-A; HLA-A; HLAA; HLAA
Gene name synonims
HLA-A; HLA-A; HLAA; HLAA
Gene name
HLA-A; HLA-A; HLAA; HLAA
Purification method
Affinity Purified by Protein G affinity chromatography.
Form/Appearance
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with FITC.
Description
Antigens are peptides or recombinant or native dependent on the production method.
Species reactivity
Human (Homo sapiens), Monkey; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Other names
HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-68 alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68
Specificity and cross-reactivity
Recognizes the human HLA-B7 antigen. Does not crossreact with HLA-B27 or other related antigens. It can be used to distinguish true HLA-B27 positives from false HLA-B27 positives (ie HLA-B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis. Species Crossreactivity: Cynomolgus monkey; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.