Size

100ug

Catalog no#

GEN601369

Price

801 EUR

Concentration

N/A

Immunoglobulin isotype

IgG1

Clone

3H1050

Category

Antibodies

Clonality

Monoclonal

Conjugation

Anti-FITC Antibody

Subcategory

Mnoclonal antibodies

Host organism

Mouse (Mus musculus)

Source organism

Human (Homo sapiens)

Tested applications:

Flow Cytometry (FC/FACS)

Also known as

CD18, Activation Epitope

Other names

CD18; N/A; CD18; N/A; N/A

Other gene names

LOC100304487; N/A; N/A; N/A

Gene name synonims

LOC100304487; N/A; N/A; N/A

Gene name

LOC100304487; N/A; N/A; N/A

Storage and shipping

Store the antibody at -20 degrees Celsius.

Purification method

Affinity Purified by Protein G affinity chromatography.

Description

Antigens are peptides or recombinant or native dependent on the production method.

Form/Appearance

Purified IgG conjugated to FluoresceIsothiocyanate Isomer 1 (FITC). Supplied as a liquid, 0.5% BSA, 0.1% sodium azide.

Species reactivity

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Specificity and cross-reactivity

Recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone 3H1050 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by 3H1050 is also exposed during dissociation of the CD11/CD18 by low pH.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Properties

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.